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Immunohistochemical staining of human cerebral cortex shows distinct cytoplasmic positivity in subsets of neuronal cells.
Immunohistochemical staining of human cerebral cortex shows distinct cytoplasmic positivity in subsets of neuronal cells.
Immunohistochemical staining of human cerebral cortex shows distinct cytoplasmic positivity in subsets of neuronal cells.

Anti-NEDD4 Antibody

HPA039883
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNEDD4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NEDD4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4734
  • Target name
    NEDD4
  • Target description
    NEDD4 E3 ubiquitin protein ligase
  • Target synonyms
    NEDD4-1, RPF1, E3 ubiquitin-protein ligase NEDD4, HECT-type E3 ubiquitin transferase NEDD4, cell proliferation-inducing gene 53 protein, neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase, receptor-potentiating factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46934
  • Protein Name
    E3 ubiquitin-protein ligase NEDD4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NEDD4 using anti-NEDD4 antibody (A00984-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD4 antigen affinity purified polyclonal antibody (Catalog # A00984-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEDD4 at approximately 149KD. The expected band size for NEDD4 is at 149KD.
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