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Immunohistochemical staining of human appendix shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human appendix shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human appendix shows cytoplasmic positivity in glandular cells.

Anti-NHSL2 Antibody

HPA049771
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNHSL2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NHSL2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID340527
  • Target name
    NHSL2
  • Target description
    NHS like 2
  • Target synonyms
    NHS-like protein 2, TPT1-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5HYW2
  • Protein Name
    NHS-like protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Immunohistochemical staining of human appendix shows cytoplasmic positivity in glandular cells.
Product group Antibodies
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Figure 1. Western blot analysis of NHSL2 using anti-NHSL2 antibody (A18099). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NHSL2 antigen affinity purified polyclonal antibody (Catalog # A18099) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NHSL2 at approximately 133 kDa. The expected band size for NHSL2 is at 133 kDa.
Product group Antibodies
Boster Bio
Anti-NHSL2 Antibody Picoband(r)A18099-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetNHSL2
  • SizePrice