Anti-Nicotine [F018]

Absolute Antibody

Anti-Nicotine [F018]

9

This antibody reacts with nicotine. Nicotine is a plant alkaloid, found in the tobacco plant and other plants of the nightshade family. It is an addictive central nervous system (CNS) stimulant that causes either ganglionic stimulation in low doses or gan

The binding characterization of this antibody to nicotine was done using ELISA. The original scFv and human IgG2 version of this antibody binds (S)-(-)-nicotine with an affinity of Kd= 11.5 +/- 1.5 nM and Kd= 7.4 +/- 1.2 nM respectively. This antibody does not cross react with (-)-cotinine and acetylcholine. It was estimated that IgG2-F018 binds (-)-cotinine about 1000 fold less strongly than (S)-(-)-nicotine. The IgG2 version of this antibody is also capable of recognizing (R)-(+)-nicotine. The IgG2 version of this antibody was used to study the distribution of nicotine in plasma and brain in mice. It was reported that this antibody can reduce the entry of nicotine to the brain by more than 60% (US8344111).

ELISA

Research Use Only

Monoclonal

F018

Rabbit

IgG

This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.

Other Species

-20°C,2°C to 8°C

12352203