Anti-Nitric Oxide Synthase [3F7-B11-B5]

Absolute Antibody

Anti-Nitric Oxide Synthase [3F7-B11-B5]

7

The antibody reacted with bovine cerebellum NOS as well as with structurally different NOSs from cultured bovine aortic endothelial cells and cultured RAW 264.7 macrophages in Western blot analysis. The reactivity of the antibody was confirmed by immunohistochemical staining of rat cerebellum, arterial endothelial cells, and cultured stimulated macrophages. The antibody showed immunoreactivity within the respiratory epithelium but not in the bronchial smooth muscle (Rengasamy et al, 1994; pmid:7528982). The antibody was used for immunohistochemistry study to investigate the neuronal nitric oxide synthase immunoreactivity in cats (Papantchev et al, 2006; pmid:17120106). The antibody was used for immunohistochemical study on human colon tissue sample (Amato et al, 2014; pmid:24443715).

Western Blot, ImmunoHistoChemistry

WB, IHC

Research Use Only

Monoclonal

3F7-B11-B5

Rat

IgG2b

Nitric oxide synthase 1

This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.

Bovine, Human, Mouse, Rat

Bovine, Mouse, Rat

The original antibody was generated by immunizing mice against purified, bovine cerebellum NOS.

-20°C,2°C to 8°C

41116161