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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.

Anti-NME8 Antibody

HPA019259
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNME8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NME8
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51314
  • Target name
    NME8
  • Target description
    NME/NM23 family member 8
  • Target synonyms
    CILD6; DNAI8; epididymis secretory protein Li 99; HEL-S-99; NM23-H8; spermatid-specific thioredoxin-2; sperm-specific thioredoxin 2; SPTRX2; sptrx-2; thioredoxin domain containing 3 (spermatozoa); thioredoxin domain-containing protein 3; TXNDC3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N427
  • Protein Name
    Thioredoxin domain-containing protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA109162(NME8 Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of TXNDC3/NME8 using anti-TXNDC3/NME8 antibody (A08723-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNDC3/NME8 antigen affinity purified polyclonal antibody (A08723-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNDC3/NME8 at approximately 67 kDa. The expected band size for TXNDC3/NME8 is at 67 kDa.
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Immunofluorescent staining of human cell line HEL shows localization to cytosol.
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