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Immunohistochemical staining of human cerebral cortex shows strong nucleolar and weaker nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong nucleolar and weaker nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong nucleolar and weaker nuclear positivity in neurons.

Anti-NOP58 Antibody

HPA021062
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNOP58
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NOP58 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51602
  • Target name
    NOP58
  • Target description
    NOP58 ribonucleoprotein
  • Target synonyms
    HSPC120, NOP5, NOP5/NOP58, nucleolar protein 58, NOP58 ribonucleoprotein homolog, nucleolar protein 5, nucleolar protein NOP5/NOP58
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2X3
  • Protein Name
    Nucleolar protein 58
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NOP58 using anti-NOP58 antibody (A05070-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOP58 antigen affinity purified polyclonal antibody (Catalog # A05070-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOP58 at approximately 70 kDa. The expected band size for NOP58 is at 60 kDa.
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