Anti-NP [JS16]

Absolute Antibody

Anti-NP [JS16]

9

The antibody binds the nucleocapsid protein of SARS-CoV-2.

The specificity of the Fab and the IgG1 formats for SARS-CoV-2 NP was confirmed by ELISA analysis. The IgG1 antibody detected SARA-Cov-2 NP by western blot. Surface plasmon resonance assay was performed to evaluate the binding specificity and affinity between antibody and purified NP; the antibody showed a tight binding affinity to immobilized NP. The antibody was used as a capture antibody in sandwich ELISA, where it was used in a matched pair in conjunction with JS08, with the optimal detection limit below 0.78ng/mL. The epitope of the antibody was analyzed by competition ELISA (Zhang et al, 2020; pmid:33281824).

Western Blot, ELISA, Other Application

Research Use Only

Monoclonal

JS16

N

nucleocapsid phosphoprotein

GU280_gp10; nucleocapsid phosphoprotein

Human

IgG2

Nucleoprotein

This reformatted human antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.

Virus

-20°C,2°C to 8°C

12352203