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Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.

Anti-NPEPPS Antibody

HPA045649
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNPEPPS
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NPEPPS Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9520
  • Target name
    NPEPPS
  • Target description
    aminopeptidase puromycin sensitive
  • Target synonyms
    AAP-S, MP100, PSA, puromycin-sensitive aminopeptidase, cytosol alanyl aminopeptidase, metalloproteinase MP100
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55786
  • Protein Name
    Puromycin-sensitive aminopeptidase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NPEPPS using anti-NPEPPS antibody (A08129-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse barin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPEPPS antigen affinity purified polyclonal antibody (Catalog # A08129-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPEPPS at approximately 110 kDa. The expected band size for NPEPPS is at 110 kDa.
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