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Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.

Anti-NR1D2 Antibody

HPA054798
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNR1D2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NR1D2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9975
  • Target name
    NR1D2
  • Target description
    nuclear receptor subfamily 1 group D member 2
  • Target synonyms
    BD73, EAR-1R, REVERBB, REVERBbeta, RVR, nuclear receptor subfamily 1 group D member 2, V-erbA-related protein 1-related, orphan nuclear hormone receptor BD73, rev-erb alpha-related receptor, rev-erb-beta, rev-erba-alpha-related receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14995
  • Protein Name
    Nuclear receptor subfamily 1 group D member 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of NR1D2 using anti-NR1D2 antibody (A04958-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1D2 antigen affinity purified polyclonal antibody (Catalog # A04958-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR1D2 at approximately 68 kDa. The expected band size for NR1D2 is at 65 kDa.
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