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Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Anti-NRP1 Antibody

HPA030278
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNRP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NRP1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8829
  • Target name
    NRP1
  • Target description
    neuropilin 1
  • Target synonyms
    BDCA4; CD304; neuropilin-1; NP1; NRP; transmembrane receptor; vascular endothelial cell growth factor 165 receptor; VEGF165R
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14786
  • Protein Name
    Neuropilin-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Neuropilin 1/NRP1 using anti-Neuropilin 1/NRP1 antibody (A01324-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neuropilin 1/NRP1 antigen affinity purified polyclonal antibody (Catalog # A01324-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neuropilin 1/NRP1 at approximately 140 kDa. The expected band size for Neuropilin 1/NRP1 is at 103 kDa.
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