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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Anti-NSD3 Antibody

HPA005659
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNSD3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NSD3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54904
  • Target name
    NSD3
  • Target description
    nuclear receptor binding SET domain protein 3
  • Target synonyms
    histone-lysine N-methyltransferase NSD3; KMT3F; KMT3G; nuclear SET domain-containing protein 3; pp14328; protein whistle; WHISTLE; WHSC1L1; Wolf-Hirschhorn syndrome candidate 1-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BZ95
  • Protein Name
    Histone-lysine N-methyltransferase NSD3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NSD3 using anti-NSD3 antibody (A32443-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSD3 antigen affinity purified polyclonal antibody (Catalog # A32443-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSD3 at approximately 90 kDa. The expected band size for NSD3 is at 90 kDa.
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