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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & nuclear speckles.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & nuclear speckles.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & nuclear speckles.

Anti-NSL1 Antibody

HPA053721
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNSL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NSL1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25936
  • Target name
    NSL1
  • Target description
    NSL1 component of MIS12 kinetochore complex
  • Target synonyms
    C1orf48; DC8; kinetochore-associated protein NSL1 homolog; MIS14; NSL1, MIND kinetochore complex component, homolog; NSL1, MIS12 kinetochore complex component
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96IY1
  • Protein Name
    Kinetochore-associated protein NSL1 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NSL1 using anti-NSL1 antibody (A09341-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat RH-35 whole cell lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSL1 antigen affinity purified polyclonal antibody (A09341-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSL1 at approximately 32 kDa. The expected band size for NSL1 is at 32 kDa.
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IHC image of CSB-PA839345LA01HU diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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