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Immunohistochemical staining of human hippocampus shows moderate nuclear membraneous positivity in neurons.
Immunohistochemical staining of human hippocampus shows moderate nuclear membraneous positivity in neurons.
Immunohistochemical staining of human hippocampus shows moderate nuclear membraneous positivity in neurons.

Anti-NSMF Antibody

HPA044316
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNSMF
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NSMF Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26012
  • Target name
    NSMF
  • Target description
    NMDA receptor synaptonuclear signaling and neuronal migration factor
  • Target synonyms
    HH9, NELF, NMDA receptor synaptonuclear signaling and neuronal migration factor, nasal embryonic LHRH factor, nasal embryonic luteinizing hormone-releasing hormone factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6X4W1
  • Protein Name
    NMDA receptor synaptonuclear signaling and neuronal migration factor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NELF/NSMF using anti-NELF/NSMF antibody (A08385-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NELF/NSMF antigen affinity purified polyclonal antibody (Catalog # A08385-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NELF/NSMF at approximately 44,60 kDa. The expected band size for NELF/NSMF is at 44,60 kDa.
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