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Immunohistochemical staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.

Anti-NSUN6 Antibody

HPA045902
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNSUN6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NSUN6 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID221078
  • Target name
    NSUN6
  • Target description
    NOP2/Sun RNA methyltransferase 6
  • Target synonyms
    4933414E04Rik, ARL5B-AS1, MRT82, NOPD1, tRNA (cytosine(72)-C(5))-methyltransferase NSUN6, ARL5B antisense RNA 1, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family, member 6, NOP2/Sun RNA methyltransferase family member 6, NOP2/Sun domain family, member 6, nucleolar protein (NOL1/NOP2/sun) and PUA domains 1, putative methyltransferase NSUN6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TEA1
  • Protein Name
    tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NSUN6 using anti-NSUN6 antibody (A13404-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN6 antigen affinity purified polyclonal antibody (A13404-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSUN6 at approximately 52 kDa. The expected band size for NSUN6 is at 52 kDa.
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