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Immunohistochemical staining of human intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human intestine shows strong cytoplasmic positivity in glandular cells.

Anti-NT5C Antibody

HPA023632
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetNT5C
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NT5C
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID30833
  • Target name
    NT5C
  • Target description
    5', 3'-nucleotidase, cytosolic
  • Target synonyms
    5' nucleotidase, deoxy (pyrimidine), cytosolic type C; 5'(3')-deoxyribonucleotidase, cytosolic type; cdN; cytosolic 5',3'-pyrimidine nucleotidase; deoxy-5'-nucleotidase 1; DNT; DNT1; dNT-1; epididymis luminal protein 74; epididymis secretory sperm binding protein; HEL74; P5N2; PN-I; PN-II; UMPH2; uridine 5'-monophosphate phosphohydrolase 2; uridine 5-prime monophosphate hydrolase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TCD5
  • Protein Name
    5'(3')-deoxyribonucleotidase, cytosolic type
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NT5C using anti-NT5C antibody (A10650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human U20S whole cell lysates, Lane 8: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT5C antigen affinity purified polyclonal antibody (Catalog # A10650-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NT5C at approximately 24 kDa. The expected band size for NT5C is at 24 kDa.
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