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Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.

Anti-NT5C1A Antibody

HPA054158
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNT5C1A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NT5C1A Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84618
  • Target name
    NT5C1A
  • Target description
    5'-nucleotidase, cytosolic IA
  • Target synonyms
    CN-I, CN-IA, CN1, CN1A, CNI, cytosolic 5'-nucleotidase 1A, 5'-deoxynucleotidase, AMP-specific 5'-NT, cytosolic 5' nucleotidase, type 1A, cytosolic 5'-nucleotidase IA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BXI3
  • Protein Name
    Cytosolic 5'-nucleotidase 1A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of NT5C1A using anti-NT5C1A antibody (A12036-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT5C1A antigen affinity purified polyclonal antibody (Catalog # A12036-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NT5C1A at approximately 41 kDa. The expected band size for NT5C1A is at 41 kDa.
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