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Immunohistochemical staining of human Heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human Heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human Heart muscle shows strong cytoplasmic positivity in cardiomyocytes.

Anti-NT5DC2 Antibody

HPA050683
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetNT5DC2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NT5DC2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64943
  • Target name
    NT5DC2
  • Target description
    5'-nucleotidase domain containing 2
  • Target synonyms
    5'-nucleotidase domain-containing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H857
  • Protein Name
    5'-nucleotidase domain-containing protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NT5DC2 using anti-NT5DC2 antibody (A16483-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT5DC2 antigen affinity purified polyclonal antibody (Catalog # A16483-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NT5DC2 at approximately 61 kDa. The expected band size for NT5DC2 is at 61 kDa.
Product group Antibodies
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Anti-NT5DC2 Antibody Picoband(r)A16483-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetNT5DC2
  • SizePrice