Anti-NTF2 [4F5]

Absolute Antibody

Anti-NTF2 [4F5]

7

This antibody is specific for NTF2. Its reactivity in humans, mice, rats, monkeys, hamsters, chickens and xenopus have been proven. The epitope of this antibody lies in the extreme C terminus of the NTF2 protein. NTF2 mediates the import of GDP-bound RAN

To characterize this antibody, a western blot on recombinant NTF2 protein was preformed using the mouse version of this antibody. Further, an ELISA on wild-type and C-terminal truncation mutants was preformed using this antibody. It only detected the wild-type protein not the C-terminal truncated proteins. Finally, the distribution of ran was determined in living BHK21 cells in the presence of this antibody. Normally ran is nuclear but with this antibody ran is redistributed to the cytoplasm (Steggerda et al, 2000; pmid:10679025).

Functional Assay, Western Blot, ELISA

WB; ELISA; functional assay

Research Use Only

Monoclonal

4F5

NUTF2

nuclear transport factor 2

NTF2; NTF-2; nuclear transport factor 2; placental protein 15; PP15

Mouse

IgG2b

Nuclear transport factor 2

Chicken, Hamster, Human, Monkey, Mouse, Rat, Xenopus

Human, Mouse, Rat, Monkey, Hamster, Chicken, Xenopus

The mouse version of this antibody was raised by immunizing mice with human NTF2.

-20°C,2°C to 8°C

12352203