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Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity.

Anti-NUDCD1 Antibody

HPA023183
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetNUDCD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NUDCD1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84955
  • Target name
    NUDCD1
  • Target description
    NudC domain containing 1
  • Target synonyms
    CML66, OVA66, nudC domain-containing protein 1, chronic myelogenous leukemia tumor antigen 66, tumor antigen CML66
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96RS6
  • Protein Name
    NudC domain-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NUDCD1 using anti-NUDCD1 antibody (A10778-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human U20S whole cell lysates, Lane 9: rat C6 whole cell lysates, Lane 10: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDCD1 antigen affinity purified polyclonal antibody (Catalog # A10778-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDCD1 at approximately 67 kDa. The expected band size for NUDCD1 is at 67 kDa.
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