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Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.

Anti-NUDT12 Antibody

HPA045449
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNUDT12
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NUDT12
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83594
  • Target name
    NUDT12
  • Target description
    nudix hydrolase 12
  • Target synonyms
    NAD-capped RNA hydrolase NUDT12, NADH pyrophosphatase NUDT12, deNADding enzyme NUDT12, nucleoside diphosphate linked moiety X-type motif 12, nucleoside diphosphate-linked moiety X motif 12, nudix (nucleoside diphosphate linked moiety X)-type motif 12, nudix motif 12, peroxisomal NADH pyrophosphatase NUDT12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BQG2
  • Protein Name
    Peroxisomal NADH pyrophosphatase NUDT12
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NUDT12 using anti-NUDT12 antibody (A13426-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT12 antigen affinity purified polyclonal antibody (A13426-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT12 at approximately 47 kDa. The expected band size for NUDT12 is at 52 kDa.
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