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Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Anti-NUP210 Antibody

HPA066888
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNUP210
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NUP210
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23225
  • Target name
    NUP210
  • Target description
    nucleoporin 210
  • Target synonyms
    GP210; nuclear envelope pore membrane protein POM 210; nuclear pore membrane glycoprotein 210; nuclear pore protein gp210; nucleoporin 210kDa; nucleoporin Nup210; POM210; pore membrane protein of 210 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TEM1
  • Protein Name
    Nuclear pore membrane glycoprotein 210
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GP210/NUP210 using anti-GP210/NUP210 antibody (A05308). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GP210/NUP210 antigen affinity purified polyclonal antibody (Catalog # A05308) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GP210/NUP210 at approximately 250 kDa. The expected band size for GP210/NUP210 is at 205 kDa.
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