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Immunohistochemical staining of human placenta shows moderate to strong nuclear membrane positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate to strong nuclear membrane positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate to strong nuclear membrane positivity in trophoblastic cells.

Anti-NUP54 Antibody

HPA035929
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNUP54
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NUP54
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID53371
  • Target name
    NUP54
  • Target description
    nucleoporin 54
  • Target synonyms
    54 kDa nucleoporin; nucleoporin 54kD; nucleoporin 54kDa; nucleoporin p54
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z3B4
  • Protein Name
    Nucleoporin p54
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUP54 using anti-NUP54 antibody (A10830-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP54 antigen affinity purified polyclonal antibody (Catalog # A10830-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP54 at approximately 56 kDa. The expected band size for NUP54 is at 56 kDa.
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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
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