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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.

Anti-OAT-25ul

HPA064742
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OAT-25ul
  • Delivery Days Customer
    6
  • Applications
    ImmunoCytoChemistry
  • Applications Supplier
    ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.05
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04181
  • Protein Name
    Ornithine aminotransferase, mitochondrial
  • Scientific Description
    Rabbit Polyclonal Anti-OAT Antibody against Human ornithine aminotransferase. Validated for Immunofluorescence
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ornithine Aminotransferase/OAT using anti-Ornithine Aminotransferase/OAT antibody (A01126-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human U20S whole cell lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ornithine Aminotransferase/OAT antigen affinity purified polyclonal antibody (Catalog # A01126-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ornithine Aminotransferase/OAT at approximately 49 kDa. The expected band size for Ornithine Aminotransferase/OAT is at 49 kDa.
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