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Immunohistochemical staining of human breast cancer shows membranous immunoreactivity in tumor cells.
Immunohistochemical staining of human breast cancer shows membranous immunoreactivity in tumor cells.
Immunohistochemical staining of human breast cancer shows membranous immunoreactivity in tumor cells.

Anti-OCLN Antibody

AMAB90890
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetOCLN
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OCLN
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL1567
  • Conjugate
    Unconjugated
  • Gene ID100506658
  • Target name
    OCLN
  • Target description
    occludin
  • Target synonyms
    BLCPMG; occludin; phosphatase 1, regulatory subunit 115; PPP1R115; PTORCH1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ16625
  • Protein Name
    Occludin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human stomach shows moderate membranous positivity in glandular cells.
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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: OCLN antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 53, 55, 32, 24, 9 kDa Observed band size: 60 kDa
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Figure 1. Western blot analysis of Occludin/OCLN using anti-Occludin/OCLN antibody (A01246-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Occludin/OCLN antigen affinity purified polyclonal antibody (Catalog # A01246-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Occludin/OCLN at approximately 65KD. The expected band size for Occludin/OCLN is at 59KD.
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