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Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity combined with staining of luminal membranes in glandular cells.
Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity combined with staining of luminal membranes in glandular cells.
Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity combined with staining of luminal membranes in glandular cells.

Anti-ODF2L Antibody

HPA028020
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetODF2L
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ODF2L Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57489
  • Target name
    ODF2L
  • Target description
    outer dense fiber of sperm tails 2 like
  • Target synonyms
    protein BCAP, ODF2-like protein, basal body centriole-associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULJ1
  • Protein Name
    Protein BCAP
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ODF2L using anti-ODF2L antibody (A16444-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF2L antigen affinity purified polyclonal antibody (Catalog # A16444-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODF2L at approximately 65 kDa. The expected band size for ODF2L is at 74 kDa.
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