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Immunohistochemical staining of human small intestine shows strong luminal membranous positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong luminal membranous positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong luminal membranous positivity in glandular cells.

Anti-OGFOD3 Antibody

HPA052099
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetOGFOD3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OGFOD3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79701
  • Target name
    OGFOD3
  • Target description
    2-oxoglutarate and iron dependent oxygenase domain containing 3
  • Target synonyms
    2-oxoglutarate and iron-dependent oxygenase domain-containing protein 3; C17orf101; PKHD domain-containing transmembrane protein C17orf101
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6PK18
  • Protein Name
    2-oxoglutarate and iron-dependent oxygenase domain-containing protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OGFOD3 using anti-OGFOD3 antibody (A15361-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGFOD3 antigen affinity purified polyclonal antibody (Catalog # A15361-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OGFOD3 at approximately 36 kDa. The expected band size for OGFOD3 is at 36 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetOGFOD3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
TargetOGFOD3
  • SizePrice
Atlas Antibodies
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