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Immunohistochemical staining of human placenta shows nuclear positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows nuclear positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows nuclear positivity in trophoblastic cells.

Anti-OLAH Antibody

HPA037947
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetOLAH
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OLAH
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55301
  • Target name
    OLAH
  • Target description
    oleoyl-ACP hydrolase
  • Target synonyms
    augmented in rheumatoid arthritis 1; AURA1; oleoyl-acyl-carrier-protein hydrolase; S-acyl fatty acid synthase thioesterase, medium chain; SAST; TE2; THEDC1; thioesterase 2; thioesterase domain containing 1; thioesterase domain-containing protein 1; thioesterase II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NV23
  • Protein Name
    S-acyl fatty acid synthase thioesterase, medium chain
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OLAH using anti-OLAH antibody (A05069-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLAH antigen affinity purified polyclonal antibody (Catalog # A05069-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLAH at approximately 36 kDa. The expected band size for OLAH is at 35 kDa.
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