Anti-Oligomeric alpha-synuclein [D5 (D5E)], Human IgG1, Fc Silent(tm), kappa

Absolute Antibody

Anti-Oligomeric alpha-synuclein [D5 (D5E)], Human IgG1, Fc Silent(tm), kappa

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This antibody recognizes the early-stage oligomeric morphological form of human alpha-synuclein. Alpha-synuclein is a small 140 amino acid presynaptic neuronal protein, a major component of Lewy bodies and a member of the synuclein family. Misfolding, abn

The binding of this antibody to oligomeric forms of alpha-synuclein was confirmed using ELISA. This antibody is not capable of recognizing monomeric and fibrillar forms of alpha-synuclein. This antibody could also recognize molecular masses of about 29 and 56 kDa corresponding to dimeric and tetrameric forms of alpha-syn form in a western blot (PMID: 17391701; 19141614). The scFv form of this antibody binds only to an oligomeric form of alpha-synuclein and inhibits both aggregation and toxicity of alpha-synuclein in vitro (PMID: 17391701). This antibody could recognize the early stage alpha-synuclein aggregates occurring during (4-10 day) time points (PMID: 19141614). Intracellular expression of scFv version of this antibody containing a non-conventional secretion signal sequence could successfully bind their cytosolic targets and secrete them from a mammalian cell. It was further demonstrated that secretion of scFv bound oligomeric alpha-syn aggregates provides complete protection from a-syn-induced toxicity (PMID: 19394405). A fusion protein comprising D5 scFv, cell penetrating peptide like penetratin were found to be effective in Lewy body disease. It specifically targeted alpha-syn oligomers and reduced the accumulation of alpha-syn and ameliorated functional deficits when delivered late in disease development (PMID: 27606342).

Functional Assay, Western Blot, ELISA

Research Use Only

Monoclonal

D5 (D5E)

SNCA

synuclein alpha

alpha-synuclein; I+/--synuclein; NACP; non A-beta component of AD amyloid; PARK1; PARK4; PD1; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); truncated alpha synuclein

Human

IgG1

Alpha-synuclein

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human scFv format, created for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203