Anti-OPGbp [AT]

Absolute Antibody

Anti-OPGbp [AT]

7

The specificity of the original format of the antibody was confirmed by ELISA analysis. The binding affinity of the Fab and IgG1 fragments of the antibody to OPGbp was measured by surface plasmon resonance (Kd~ 140 nM and 0.33-0.43 nM respectively). The interaction of human OPGbp and its receptor ODAR was measured in a fluorescence energy transfer (FRET) assay. The Fab fragment of the antibody inhibited OPGbp binding to soluble ODAR-Fc fusion with an IC50 of 550 nM. Bone Marrow Assay using the Fab fragment of the antibody gave a 50% decrease (IC50) at 57.8 nM. In RAW Cell Assay the Fab fragment of the antibody showed an IC50 of 15 microg/ml, 300 nM. In RAW Cell Assay, the 3 full length versions of the antibody AT405, AT406 and AT407 showed IC50 of 20.1 nM, 60.3 nM & 21.4 nM, respectively. AT405 or AT407 were tested in murine bone marrow assay, showing IC50 of 2.15 ug/ml (14.4 nM), and 1.85 ug/ml (12.5 nM), respectively. AT406 was tested in the bone marrow coculture assay and inhibited TRAP formation with an IC50 of 2.35 microg/ml (14.4 nM) (US20140147444).

ELISA, Neutralisation/Blocking, Other Application

Inhibition; ELISA; SPR

Research Use Only

Tumor necrosis factor receptor superfamily member 11B

-20°C,2°C to 8°C

12352203