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Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in purkinje cells.

Anti-OPLAH Antibody

HPA028260
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOPLAH
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OPLAH Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26873
  • Target name
    OPLAH
  • Target description
    5-oxoprolinase, ATP-hydrolysing
  • Target synonyms
    5-Opase, OPLA, OPLAHD, 5-oxoprolinase, 5-oxo-L-prolinase, pyroglutamase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14841
  • Protein Name
    5-oxoprolinase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OPLAH using anti-OPLAH antibody (A11695-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPLAH antigen affinity purified polyclonal antibody (Catalog # A11695-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPLAH at approximately 140 kDa. The expected band size for OPLAH is at 135-140 kDa.
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