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Immunohistochemical staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.

Anti-OPRM1 Antibody

HPA014509
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOPRM1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OPRM1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4988
  • Target name
    OPRM1
  • Target description
    opioid receptor mu 1
  • Target synonyms
    LMOR, M-OR-1, MOP, MOR, MOR1, OPRM, mu-type opioid receptor, mu opiate receptor, mu opioid receptor hMOR-1a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35372
  • Protein Name
    Mu-type opioid receptor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OPRM1 using anti-OPRM1 antibody (A00737-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPRM1 antigen affinity purified polyclonal antibody (Catalog # A00737-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPRM1 at approximately 70 kDa. The expected band size for OPRM1 is at 45 kDa.
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