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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.

Anti-ORC2 Antibody

HPA073881
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, Chip-exoSeq
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetORC2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ORC2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, Chip-exoSeq
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4999
  • Target name
    ORC2
  • Target description
    origin recognition complex subunit 2
  • Target synonyms
    ORC2L; origin recognition complex protein 2 homolog; origin recognition complex subunit 2; origin recognition complex, subunit 2 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13416
  • Protein Name
    Origin recognition complex subunit 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ORC2 using anti-ORC2 antibody (A04350-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A2780 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORC2 antigen affinity purified polyclonal antibody (Catalog # A04350-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORC2 at approximately 74 kDa. The expected band size for ORC2 is at 66 kDa.
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