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Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Anti-ORC5 Antibody

HPA019730
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetORC5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ORC5 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5001
  • Target name
    ORC5
  • Target description
    origin recognition complex subunit 5
  • Target synonyms
    ORC5L, ORC5P, ORC5T, PPP1R117, origin recognition complex subunit 5, protein phosphatase 1, regulatory subunit 117
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43913
  • Protein Name
    Origin recognition complex subunit 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ORC5L/ORC5 using anti-ORC5L/ORC5 antibody (A03676-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORC5L/ORC5 antigen affinity purified polyclonal antibody (Catalog # A03676-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORC5L/ORC5 at approximately 50 kDa. The expected band size for ORC5L/ORC5 is at 50 kDa.
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TargetORC5
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