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Immunohistochemical staining of human caudate nucleus shows moderate membranous positivity in neuropil.
Immunohistochemical staining of human caudate nucleus shows moderate membranous positivity in neuropil.
Immunohistochemical staining of human caudate nucleus shows moderate membranous positivity in neuropil.

Anti-OTOF Antibody

HPA012410
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOTOF
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OTOF Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9381
  • Target name
    OTOF
  • Target description
    otoferlin
  • Target synonyms
    AUNB1, DFNB6, DFNB9, FER1L2, NSRD9, otoferlin, fer-1-like family member 2, fer-1-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HC10
  • Protein Name
    Otoferlin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Otoferlin using anti-Otoferlin antibody (PB9616). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Otoferlin antigen affinity purified polyclonal antibody (Catalog # PB9616) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Otoferlin at approximately 227 kDa. The expected band size for Otoferlin is at 227 kDa.
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