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Anti-OX40L [OX-89]

AB00564-6.1-BT
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
Product group Antibodies
TargetTnfsf4
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-OX40L [OX-89]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody binds mouse CD134L, and has been shown to bind activated B cells and DCs, as well as CD134L-transfected fibroblast cells, though not activated T cells, in labelling and immunohistochemical analysis (Malmstrom, 2001). In addition, this antibody is able to partially block the interaction between CD134 and CD134L. This blocking effect could be responsible for the ability of this antibody to prevent T cell accumulation in the intestine of T cell-restored SCID mice and inhibits the development of Th1-mediated colitis.
  • Applications
    Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX-89
  • Gene ID22164
  • Target name
    Tnfsf4
  • Target description
    tumor necrosis factor (ligand) superfamily, member 4
  • Target synonyms
    Ath; Ath-; Ath1; Ath-1; atherosclerosis 1; CD134; CD134L; gp3; gp34; OX4; OX40 ligand; Ox40l; OX-40L; tax-transcriptionally activated glycoprotein 1 ligand; Tnlg2b; tumor necrosis factor ligand 2b; tumor necrosis factor ligand superfamily member 4; Txgp; TXGP1; Txgp1l
  • Host
    Rat
  • Isotype
    IgG1
  • Protein IDP43488
  • Protein Name
    Tumor necrosis factor ligand superfamily member 4
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD252 using anti-CD252 antibody (A02554-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse CD252 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD252 antigen affinity purified polyclonal antibody (Catalog # A02554-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD252 at approximately 21-25KD. The expected band size for CD252 is at 18KD.
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