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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.

Anti-OXSR1 Antibody

HPA008237
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetOXSR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OXSR1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9943
  • Target name
    OXSR1
  • Target description
    oxidative stress responsive kinase 1
  • Target synonyms
    OSR1; oxidative stress responsive 1; oxidative stress-responsive 1 protein; serine/threonine-protein kinase OSR1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95747
  • Protein Name
    Serine/threonine-protein kinase OSR1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OXSR1 using anti-OXSR1 antibody (A05141-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXSR1 antigen affinity purified polyclonal antibody (Catalog # A05141-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXSR1 at approximately 58 kDa. The expected band size for OXSR1 is at 58 kDa.
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