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Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.

Anti-P2RX7 Antibody

HPA034968
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetP2RX7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-P2RX7 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5027
  • Target name
    P2RX7
  • Target description
    purinergic receptor P2X 7
  • Target synonyms
    P2X7, P2X purinoceptor 7, ATP receptor, P2X7 receptor, P2Z receptor, purinergic receptor P2X, ligand gated ion channel, 7, purnergic receptor P2X 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99572
  • Protein Name
    P2X purinoceptor 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of P2X7/P2RX7 using anti-P2X7/P2RX7 antibody (A01208-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2X7/P2RX7 antigen affinity purified polyclonal antibody (Catalog # A01208-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2X7/P2RX7 at approximately 70 kDa. The expected band size for P2X7/P2RX7 is at 70 kDa.
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