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Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.

Anti-PAAF1 Antibody

HPA039952
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPAAF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PAAF1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80227
  • Target name
    PAAF1
  • Target description
    proteasomal ATPase associated factor 1
  • Target synonyms
    PAAF, Rpn14, WDR71, proteasomal ATPase-associated factor 1, WD repeat domain 71, WD repeat-containing protein 71
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BRP4
  • Protein Name
    Proteasomal ATPase-associated factor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PAAF1 using anti-PAAF1 antibody (A12843-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAAF1 antigen affinity purified polyclonal antibody (Catalog # A12843-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAAF1 at approximately 42 kDa. The expected band size for PAAF1 is at 42 kDa.
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Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.
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