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Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.

Anti-PAQR6 Antibody

HPA073505
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPAQR6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PAQR6
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79957
  • Target name
    PAQR6
  • Target description
    progestin and adipoQ receptor family member 6
  • Target synonyms
    PRdelta, membrane progestin receptor delta, mPR delta, membrane progesterone P4 receptor delta, membrane progesterone receptor delta, progesterone and adipoQ receptor family member 6, progestin and adipoQ receptor family member VI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6TCH4
  • Protein Name
    Membrane progestin receptor delta
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PAQR6 using anti-PAQR6 antibody (A14355-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAQR6 antigen affinity purified polyclonal antibody (Catalog # A14355-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAQR6 at approximately 38 kDa. The expected band size for PAQR6 is at 38 kDa.
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