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Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.

Anti-PARVA Antibody

HPA005964
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPARVA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PARVA
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55742
  • Target name
    PARVA
  • Target description
    parvin alpha
  • Target synonyms
    CH-ILKBP, MXRA2, alpha-parvin, actopaxin, calponin-like integrin-linked kinase-binding protein, matrix-remodeling-associated protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Parvin alpha using anti-Parvin alpha antibody (PB9555). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: SW620 Whole Cell Lysate at 40ug, Lane 4: HEPA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Parvin alpha antigen affinity purified polyclonal antibody (Catalog # PB9555) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Parvin alpha at approximately 42 kDa. The expected band size for Parvin alpha is at 42 kDa.
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