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Immunohistochemical staining of human kidney shows strong nuclear and cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong nuclear and cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong nuclear and cytoplasmic positivity in cells in tubules.

Anti-PATZ1 Antibody

HPA047893
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry, Chip-exoSeq
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPATZ1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PATZ1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry, Chip-exoSeq
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23598
  • Target name
    PATZ1
  • Target description
    POZ/BTB and AT hook containing zinc finger 1
  • Target synonyms
    BTB-POZ domain zinc finger transcription factor; dJ400N23; MAZR; MAZ-related factor; PATZ; POZ-, AT hook-, and zinc finger-containing protein 1; POZ-AT hook-zinc finger protein; protein kinase A RI subunit alpha-associated protein; RIAZ; ZBTB19; zinc finger and BTB domain-containing protein 19; zinc finger protein 278; zinc finger sarcoma gene protein; ZNF278; ZSG
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HBE1
  • Protein Name
    POZ-, AT hook-, and zinc finger-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PATZ1 using anti-PATZ1 antibody (A06823-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PATZ1 antigen affinity purified polyclonal antibody (Catalog # A06823-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PATZ1 at approximately 70 kDa. The expected band size for PATZ1 is at 74 kDa.
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