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Immunohistochemical staining of human skin shows strong positivity in nucleoli in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong positivity in nucleoli in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong positivity in nucleoli in squamous epithelial cells.

Anti-PAX3 Antibody

HPA063659
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPAX3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PAX3
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5077
  • Target name
    PAX3
  • Target description
    paired box 3
  • Target synonyms
    CDHS; HUP2; paired box homeotic gene 3; paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; transcriptional factor PAX3; WS1; WS3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23760
  • Protein Name
    Paired box protein Pax-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: A2780 whole cell lysate, A375 whole cell lysate All lanes: PAX3 antibody at 5.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 25, 23, 45, 46, 54, 56 kDa Observed band size: 53 kDa
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Figure 1. Western blot analysis of PAX3 using anti-PAX3 antibody (A00285-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX3 antigen affinity purified polyclonal antibody (Catalog # A00285-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX3 at approximately 53KD. The expected band size for PAX3 is at 53KD.
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