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Anti-PAX4 Antibody

Anti-PAX4 Antibody

A14747
Antibodies.com
ApplicationsWestern Blot
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-PAX4 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to PAX4.
  • UNSPSC
    12352203

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Western blot All lanes: Paired box protein Pax-4 antibody at 6ug/ml Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Mouse pancreatic Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 38, 30 kDa Observed band size: 38 kDa
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Figure 1. Western blot analysis of PAX4 using anti-PAX4 antibody (A03165-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX4 antigen affinity purified polyclonal antibody (Catalog # A03165-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX4 at approximately 38 kDa. The expected band size for PAX4 is at 38 kDa.
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ELISA analysis of antigen using GTX60387 PAX4 antibody [3C12]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
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