Figure 1. Western blot analysis of PAX5 using anti-PAX5 antibody (M00669-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PAX5 antigen affinity purified monoclonal antibody (Catalog # M00669-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PAX5 at approximately 45KD. The expected band size for PAX5 is at 45KD.
Figure 1. Western blot analysis of PAX5 using anti-PAX5 antibody (M00669-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PAX5 antigen affinity purified monoclonal antibody (Catalog # M00669-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PAX5 at approximately 45KD. The expected band size for PAX5 is at 45KD.
Anti-PAX5 Antibody Picoband(r) (monoclonal, 6I4)
M00669-2-APC
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPAX5
Overview
- SupplierBoster Bio
- Product NameAnti-PAX5 Antibody Picoband(r) (monoclonal, 6I4)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID6I4
- ConjugateAPC (Allophycocyanin)
- Gene ID5079
- Target namePAX5
- Target descriptionpaired box 5
- Target synonymsALL3; B-cell lineage specific activator; BSAP; paired box homeotic gene 5; paired box protein Pax-5; paired domain gene 5; transcription factor PAX 5
- HostMouse
- IsotypeIgG2b
- Protein IDQ02548
- Protein NamePaired box protein Pax-5
- Scientific DescriptionBoster Bio Anti-PAX5 Antibody Picoband® (monoclonal, 6I4) catalog # M00669-2. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203