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Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in purkinje cells.

Anti-PCDHA12 Antibody

HPA035812
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPCDHA12
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PCDHA12
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56137
  • Target name
    PCDHA12
  • Target description
    protocadherin alpha 12
  • Target synonyms
    KIAA0345-like 2; PCDH-ALPHA12; PCDH-alpha-12; protocadherin alpha-12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UN75
  • Protein Name
    Protocadherin alpha-12
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PCDHA12 using anti-PCDHA12 antibody (A15869-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCDHA12 antigen affinity purified polyclonal antibody (Catalog # A15869-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCDHA12 at approximately 120 kDa. The expected band size for PCDHA12 is at 102 kDa.
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