Anti-PCNA Mouse mAb

PTM BIO

Anti-PCNA Mouse mAb

5

Western Blot, ImmunoHistoChemistry Paraffin

WB, IHC-P

Research Use Only

Monoclonal

10937

Unconjugated

Mouse

IgG

Proliferating cell nuclear antigen

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-Phosphorylateddiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Stable for 12 months from date of receipt/reconstitution.

Human, Mouse, Rat

Protein G purified

Store at -20°C. Avoid freeze/thaw cycles.

12352203