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Anti-PCSK9 [mAb1]

Ab02935-10.0
Absolute Antibody
ApplicationsFunctional Assay, ELISA
Product group Antibodies
TargetPCSK9
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-PCSK9 [mAb1]
  • Delivery Days Customer
    7
  • Application Supplier Note
    Proprotein convertase subtilisin/kexin type 9 (PCSK9) regulates serum LDL cholesterol (LDL-C) by interacting with the LDL receptor (LDLR) and is an attractive therapeutic target for LDL-C lowering. This antibody blocks access to an area bearing several naturally occurring gain-of-function and loss-of-function mutations. This antibody does not completely inhibit binding of PCSK9 to epidermal growth factor-like repeat A, it partially reverses PCSK9-induced reduction of the LDLR and LDL cholesterol uptake in a cellular assay. This antibody was also reported to be effective in lowering serum levels of LDL cholesterol in cynomolgus monkeys in vivo (PMID: 24252255).
  • Applications
    Functional Assay, ELISA
  • Applications Supplier
    ELISA; functional assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    mAb1
  • Gene ID255738
  • Target name
    PCSK9
  • Target description
    proprotein convertase subtilisin/kexin type 9
  • Target synonyms
    FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, proprotein convertase subtilisin/kexin type 9, convertase subtilisin/kexin type 9 preproprotein, neural apoptosis regulated convertase 1, subtilisin/kexin-like protease PC9
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDQ8NBP7
  • Protein Name
    Proprotein convertase subtilisin/kexin type 9
  • Reactivity Supplier
    Human, cynomolgus monkeys
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCSK9 using anti-PCSK9 antibody (A00085-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK9 antigen affinity purified polyclonal antibody (Catalog # A00085-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK9 at approximately 66 kDa, 80 kDa (precursor). The expected band size for PCSK9 is at 72 kDa.
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