Anti-PD-L1 [JC071]

Absolute Antibody

Anti-PD-L1 [JC071]

7

This antibody is recommended for detection of canine PD-L1. This antibody successfully detected canine PD-L1 in a western blot assay. JC071 was used to determine the increased PD-1 expression on canine PBMCs stimulated with peptidoglycan or lipopolysaccharide using flow cytometry. JC071 was also able to detect recombinant PD-L1 expressed on DH82 cell line both with and without in vitro activation using IFN-gamma in flow cytometry. It also recognized recombinant canine PD-L1 expressed on CHO cells in flow cytometry. The ability of the antibody to block interactions between PD-1 and PD-L1 was checked using ELISA. It is known that blockade of PD-L1 and PD-1 interactions causes increase in Ifn-gamma level. JC071 successfully blocked PD-L1 and amplified IFN-gamma production in culture by approximately 6-fold. Thus, JC071 alleviated T-cell suppression and led to enhanced functional activity of T cells in culture and have potential application as an immunotherapy reagent for cancer treatment (PMID: 32614928).

Flow Cytometry, Western Blot, ELISA, Neutralisation/Blocking

FC, WB, ELISA, Block

Research Use Only

CD274 molecule

-20°C,2°C to 8°C

12352203