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Immunohistochemical staining of human Heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human Heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human Heart muscle shows strong cytoplasmic positivity in cardiomyocytes.

Anti-PDE4D Antibody

HPA045895
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPDE4D
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDE4D
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5144
  • Target name
    PDE4D
  • Target description
    phosphodiesterase 4D
  • Target synonyms
    ACRDYS2; cAMP-specific 3',5'-cyclic phosphodiesterase 4D; cAMP-specific phosphodiesterase PDE4D6; DPDE3; HSPDE4D; PDE43; PDE4DN2; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila); STRK1; testicular tissue protein Li 136
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08499
  • Protein Name
    cAMP-specific 3',5'-cyclic phosphodiesterase 4D
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA048149(PDE4D Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of PDE4D using anti-PDE4D antibody (A01111-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE4D antigen affinity purified polyclonal antibody (Catalog # A01111-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE4D at approximately 91KD. The expected band size for PDE4D is at 91KD.
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