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Western blot analysis in human cell line U-251 MG and human cell line A-549.
Western blot analysis in human cell line U-251 MG and human cell line A-549.
Western blot analysis in human cell line U-251 MG and human cell line A-549.

Anti-PDE4DIP Antibody

HPA008162
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDE4DIP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDE4DIP Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9659
  • Target name
    PDE4DIP
  • Target description
    phosphodiesterase 4D interacting protein
  • Target synonyms
    CMYA2, MMGL, myomegalin, cardiomyopathy-associated protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDE4DIP using anti-PDE4DIP antibody (A07608-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE4DIP antigen affinity purified polyclonal antibody (Catalog # A07608-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE4DIP at approximately 150 kDa. The expected band size for PDE4DIP is at 150 kDa.
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